Histone H2AX (9C9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54151R-100uL |
| Product Name | Histone H2AX (9C9) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide within human Histone H2A.X aa 1-50 |
| Clonality | Monoclonal |
| Clone # | 9C9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 3014 |
| Swiss Prot | P16104 |
| Synonyms | Histone H2A.X; Histone H2AX; H2a/x; H2AX; H2AFX.; H2A histone family member X. |
| Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) validated for, and at what dilution?
bsm-54151R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54151R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R)?
Synthetic peptide within human Histone H2A.X aa 1-50