RPA1 (1C2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54296R-100uL |
| Product Name | RPA1 (1C2) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein corresponding to Human RPA70 aa 1-100 |
| Clonality | Monoclonal |
| Clone # | 1C2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 6117 |
| Swiss Prot | P27694 |
| Subcellular location | Nucleus |
| Synonyms | Replication protein A 70 kDa DNA-binding subunit; Replication factor A protein 1; Single-stranded DNA-binding protein; RP-A p70; RF-A protein 1; RPA1; REPA1; RPA70 |
| Background | This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and coordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is RPA1 (1C2) Monoclonal Antibody (bsm-54296R) validated for, and at what dilution?
bsm-54296R is validated for WB (1:300-5000); IF(ICC) (1:50-200); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RPA1 (1C2) Monoclonal Antibody (bsm-54296R) react with?
Tested reactivity: Human.
How should bsm-54296R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RPA1 (1C2) Monoclonal Antibody (bsm-54296R)?
Recombinant protein corresponding to Human RPA70 aa 1-100