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H2AX | Histone H2A.X (ub-K119) Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (bsm-55852R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with H2AX | Histone H2A.X (ub-K119) monoclonal antibody, unconjugated (bsm-55852R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with H2AX | Histone H2A.X (ub-K119) monoclonal antibody, unconjugated (bsm-55852R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • ChIP
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55852r-100ul
Product Name H2AX | Histone H2A.X (ub-K119) Recombinant Antibody
Applications
WB ELISA IHC-F IP IF CHIP IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised Ubiquitylpeptide derived from human H2AX around the Ubiquitylation site of Lys119
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3014
Swiss Prot P16104
Subcellular location Nucleus
Synonyms H2A.X; H2A/X; H2AFX; Hist5-2ax; gammaH2ax; RGD1566119; H2AX_HUMAN; H2AX; Histone H2A.X; H2AX_MOUSE; H2A.X variant histone; H2A histone family, member X; H2A histone family member X; H2AX (ubiquityl-K119); H2AXK119Ub; ub-H2AX; ubiquityl-H2AX; H2AX (ubiquityl-Lys119)
Application Dilution
WB 1:500-2000
ELISA 1:5000-20000
IHC-F 1:200-800
IP 1:50-200
IF 1:200-800
ChIP 1:50-100
IHC-P 1:200-800

Frequently asked questions

Which applications is H2AX | Histone H2A.X (ub-K119) Recombinant Antibody (bsm-55852R) validated for, and at what dilution?

bsm-55852R is validated for WB (1:500-2000); ELISA (1:5000-20000); IHC-F (1:200-800); IP (1:50-200); IF (1:200-800); ChIP (1:50-100); IHC-P (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does H2AX | Histone H2A.X (ub-K119) Recombinant Antibody (bsm-55852R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55852R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise H2AX | Histone H2A.X (ub-K119) Recombinant Antibody (bsm-55852R)?

KLH conjugated Synthesised Ubiquitylpeptide derived from human H2AX around the Ubiquitylation site of Lys119

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