RNF8 Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61658r-pe-Cy5.5-100uL |
| Product Name | RNF8 Recombinant Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF8 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9025 |
| Swiss Prot | O76064 |
| Subcellular location | Nucleus |
| Synonyms | C3HC4 type zinc finger protein; RNF8. |
| Background | E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RNF8 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61658R-PE-CY5-5) validated for, and at what dilution?
BSM-61658R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF8 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61658R-PE-CY5-5) react with?
Tested reactivity: Human, Rat.
How should BSM-61658R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF8 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61658R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human RNF8
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-61658R-PE-CY5-5)?
PE-Cy5.5 on BSM-61658R-PE-CY5-5: excitation / emission 488nm/694nm.