NHEJ1 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62071r-cy5-100uL |
| Product Name | NHEJ1 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NHEJ1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79840 |
| Swiss Prot | Q9H9Q4 |
| Synonyms | Cernunno; Nhej1; Non homologous end joining factor 1; Protein cernunnos; XLF; XRCC4 like factor. |
| Background | DNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination.Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends.Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is NHEJ1 Recombinant Antibody, Cy5 Conjugated (bsm-62071R-Cy5) validated for, and at what dilution?
bsm-62071R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NHEJ1 Recombinant Antibody, Cy5 Conjugated (bsm-62071R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62071R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NHEJ1 Recombinant Antibody, Cy5 Conjugated (bsm-62071R-Cy5)?
KLH conjugated synthetic peptide derived from human NHEJ1
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62071R-Cy5)?
Cy5 on bsm-62071R-Cy5: excitation / emission 625,650nm/670nm.