CRISPR Cas9 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70054M-100uL |
| Product Name | CRISPR Cas9 Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for the Staphylococcus aureus Cas9 protein. |
| Reactivity | Others |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus. |
| Clonality | Monoclonal |
| Clone # | 6F7 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 50% glycerol and 5 mM NaN3 |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Synonyms | Cas9 antibody, CRISPR-associated endonuclease Cas9/Csn1 antibody, CRISPR-Cas9/Csn1 antibody, csn1 antibody, SpyCas9 antibody |
| Background | CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is CRISPR Cas9 Antibody (bsm-70054M) validated for, and at what dilution?
bsm-70054M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-70054M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 50% glycerol and 5 mM NaN3
What immunogen was used to raise CRISPR Cas9 Antibody (bsm-70054M)?
Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.