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\"Antigen: bs-1240P Hu Fibrinogen, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Mouse IgG (bs-0296G-HRP) at 1: 5000; \\nRead the data in MicroplateReader by 450nm. \\n\"
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with human Fibrinogen Monoclonal Antibody(bsm-1240M)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-1240M

Applications: IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Human Serum albumin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-0945m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0945m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-51054M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-0945m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-1240M-FITC

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-1240M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0945m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-1240M-Cy3

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-1240M-Cy5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-1240M-Cy7

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-1240M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-51192M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0945m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-1240M-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-0945m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-0945m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0945m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0945m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0945m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0945m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0945m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0945m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0945m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0945m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-0945m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-0945m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-0945m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-0945m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-0945m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-0945m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-0945m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0945m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-1240M-BF488

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-1240M-BF350

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-1240M-BF405

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-1240M-BF555

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-1240M-BF594

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-1240M-BF647

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-1240M-BF680

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-1240M-BF750

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-1240M-APC

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-1240M-APC-Cy7

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-1240M-APC-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-1240M-PerCP

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP