Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to a2PI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to a2PI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain a2PI, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of a2PI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity).
SERPINF2; AAP; A2-PI; API; PLI; A2AP; Alpha 2 Antiplasmin; Serpin Peptidase Inhibitor Clade F Member 2; Pigment Epithelium Derived Factor
|Kit Components||96 Wells Quantity/Size|
|Pre-coated, ready-to-use 96-well strip plate||1 plate|
|Plate sealer for 96 wells||2|
|Diluent buffer||1 bottle|
|Detection Reagent A||1 bottle|
|Detection Reagent B||1 bottle|
|TMB Substrate||1 tube|
|Stop Solution||1 tube|
|Wash Buffer (30 ℅ concentrate)||1 tube|
|Product data sheet||1 copy|
|Storage||The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C.|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level a2PI were tested 20 times on one plate, respectively.
|SENSITIVITY||The minimum detectable dose was 7.7ng/mL.|
|SPECIFICITY||This assay has high sensitivity and excellent specificity for the detection of a2PI.
No significant cross-reactivity or interference between a2PI and analogs was observed.
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between a2PI and all analogs, therefore, cross reactivity may still exist.