Mouse Thioredoxin (Trx) ELISA Kit
Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trx. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Trx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Trx, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trx in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (By similarity).
TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
|Kit Components||96 Wells Quantity/Size|
|Pre-coated, ready-to-use 96-well strip plate||1 plate|
|Plate sealer for 96 wells||2|
|Diluent buffer||1 bottle|
|Detection Reagent A||1 bottle|
|Detection Reagent B||1 bottle|
|TMB Substrate||1 tube|
|Stop Solution||1 tube|
|Wash Buffer (30 ℅ concentrate)||1 tube|
|Product data sheet||1 copy|
|Storage||The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C.|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level Trx were tested 20 times on one plate, respectively.
|SENSITIVITY||The minimum detectable dose was 21.3pg/mL.|
|SPECIFICITY||This assay has high sensitivity and excellent specificity for the detection of Trx.
No significant cross-reactivity or interference between Trx and analogs was observed.
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between Trx and all analogs, therefore, cross reactivity may still exist.