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Endothelial Cell Type Markers



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Catalog #1: bsm-10697m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-10697m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33176M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33176M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33176M-Cy5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33176M-Cy3

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33176M-Cy5.5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33176M-Cy7

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33176M-FITC

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144\/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144\/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates probed with CD144\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-22363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD144\/VE Cadherin) Polyclonal Antibody, Unconjugated (bs-22363R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-22363R

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-22363R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-22363R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41196M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41196M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41196M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41197M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41197M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41197M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2089R-PE

Applications: WB, FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bs-5450R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-5450R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-5450R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5450R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-5450R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-1618R-Cy3

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-1618R-Cy5

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-1618R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-1618R-Cy7

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-1618R-FITC

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-1618R-PE

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bs-2038r-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2038r-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2038r-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-2038r-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-3589R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-41196M-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-41196M-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-41196M-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-41196M-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41196M-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-34063r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13073R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-3731R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bs-43012R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43012R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-43012R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-1300R-BF350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-1300R-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-405