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Endothelial Cell Type Markers



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Human U937 cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:20 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
U937 lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0195R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37\u00b0C
Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

Catalog #1: bs-0646r-TR

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0586R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa cells probed with Rabbit Anti-VEGFR2 Polyclonal Antibody (bs-10412R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
Formalin-fixed and paraffin embedded mouse placenta tissue labeled with Anti-VEGFR2 Polyclonal Antibody, Unconjugated (bs-10412R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody \uff08bs-10412R\uff09 for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bs-10412r

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54\/ICAM-1 Polyclonal Antibody (bs-0608R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54\/ICAM-1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 60kD. Observed band size: 60kD.
Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Lane 1: Hela cell lysates; Lane 2: Hela KO \r\nICAM1 cell lysates probed with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0608R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Expression of cell surface marker CD34 and CD133 detected by flow cytometry
Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated(bs-2038R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3), and DAPI staining
Lane 1: human colon carcinoma Lane 2: rat kidney lysates probed with Anti CD34 Polyclonal Antibody, Unconjugated (bs-2038R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD.
25 ug total protein per lane of various lysates (see on figure) probed with CD34 polyclonal antibody, unconjugated (bs-2038R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-2038r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0468R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: FITC

Catalog #1: bs-0646r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0646r-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-10412R-FITC

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0468R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Dog

Conjugate: Biotin

Catalog #1: bs-0468R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: PE

Catalog #1: bs-10412R-PE

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-0468R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor-647

Catalog #1: bs-0468R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy5

Catalog #1: bs-0646r-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0646r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0468R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy3

Catalog #1: bs-0468R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy7

Catalog #1: bs-0646r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0195R-FITC

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-2038r-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0646r-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0646r-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-10412R-Cy5

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-2038r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0195R-Cy7

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-2038r-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0468R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor-750

Catalog #1: bs-0195R-BF647

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-0468R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Dog

Conjugate: HRP

Catalog #1: bs-10412R-Cy3

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-10412R-Cy7

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-10412R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-10412R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-2038r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0468R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Cy5.5

Catalog #1: bs-0646r-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-10412R-Cy5.5

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0195R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0195R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0195R-Cy3

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0195R-Cy5

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0195R-Cy5.5

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0195R-PE

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-2038r-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2038r-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2038r-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE