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Endothelial Cell Type Markers



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Catalog #1: bsm-0954M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (bsm-10825M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (bsm-10825M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (bsm-10825M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (bsm-10825M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-10825m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33176M

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0954M-HRP

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bsm-10825m-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-0954M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bsm-0954M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bsm-0954M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bsm-0954M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: FITC

Catalog #1: bsm-0954M-Biotin

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Lane 1: VWF protein; probed with VWF (907CT12.1.9) Monoclonal Antibody, unconjugated (bsm-51280M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with VWF (907CT12.1.9) Monoclonal Antibody (bsm-51280M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51280M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51026M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-10825m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-10825m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-0954M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy5.5

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (ascites of bsm-10697M) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-10697m

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10697m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-10697m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33176M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33176M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33176M-Cy5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33176M-Cy3

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33176M-Cy5.5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33176M-Cy7

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33176M-FITC

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-41196M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41196M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41196M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41197M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41197M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41197M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41196M-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-41196M-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-41196M-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-41196M-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41196M-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (human CD34) Monoclonal Antibody, Unconjugated (bsm-30097M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34 ) Monoclonal Antibody, Unconjugated (bsm-30097M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
TF-1 cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with human CD34 Antibody(bsm-30097M)at 1:200 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-30097m

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0954M-PE

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: PE

Catalog #1: bsm-0954M-BF350

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0954M-BF405

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0954M-BF488

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0954M-BF555

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0954M-BF594

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0954M-BF647

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0954M-BF680

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0954M-BF750

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0954M-APC

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: APC

Catalog #1: bsm-0954M-APC-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: APC-Cy5.5