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ZBTB4 Polyclonal Antibody

To determine the titer, an ELISA was performed using a serial dilution of the ZBTB4 antibody (bs-53077R). The plates were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:50,000.
To determine the titer, an ELISA was performed using a serial dilution of the ZBTB4 antibody (bs-53077R). The plates were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:50,000.
Bioss unconjugated antibody vial

Applications

  • ELISA

Reactivity

  • Human
Overview
Catalog # bs-53077R
Product Name ZBTB4 Polyclonal Antibody
Applications
ELISA
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against human ZBTB4 (zinc finger and BTB domain containing 4), using four different KLH-conjugated synthetic peptides
Clonality Polyclonal
Isotype Not Determined
Concentration Not Determined
Purification Whole antiserum from rabbit containing 0.05% azide
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 57659
Swiss Prot Q9P1Z0
Synonyms Zinc finger and BTB domain-containing protein 4, ZBTB4,
Background Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA (PubMed:16354688). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal.
Application Dilution
ELISA 1:500-1000

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