UCHL1/PGP9.5 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human astroglioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human astroglioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: 293T cell lysates; Lane 2: NCI-H1299 cell lysates; Lane 3: PC-12 cell lysates probed with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293T cell lysates; Lane 2: NCI-H1299 cell lysates; Lane 3: PC-12 cell lysates probed with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Xenopus laevis

Predicted Reactivity

Human
Mouse
Rat
Cow
Pig
Monkey

Overview
Catalog #	bsm-51558m
Product Name	UCHL1/PGP9.5 Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Xenopus laevis
Predicted Reactivity	Human, Mouse, Rat, Cow, Pig, Monkey
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human UCHL1/PGP9.5
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7345
Swiss Prot	P09936
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; Park 5; Park5; Parkinson Disease 5; PGP 9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase (neuron specific); Ubiquitin C terminal hydrolase; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH L1; UCH-L1; UCHL1; UCHL1_HUMAN.
Background	Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:20-50
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500