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25 ug total protein per lane of various lysates (see on figure) probed with AFP(A2) monoclonal antibody, unconjugated (bsm-1621M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(bsm-1621M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-1621m

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Hsp70 (2G2) Monoclonal Antibody(bsm-52241R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with HSP70 monoclonal antibody, unconjugated (bsm-52241R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HSP70 Monoclonal Antibody, Unconjugated(bsm-52241R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HSP70 Monoclonal Antibody, Unconjugated(bsm-52241R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52241r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-1622M-Cy3

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-1622M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-4792M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1621m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-1621m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-1624M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-51153M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

HeLa cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Mouse Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with HSP70 (5G10) Monoclonal Antibody, Unconjugated (bsm-33048M) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bsm-33048M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Pig

Conjugate: Unconjugated

Catalog #1: bsm-4792M-Cy3

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-4792M-Cy5

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-4792M-Cy7

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-4792M-FITC

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-4792M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-4792M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

HT29 Cell lysates probed with CEA (5F2) Monoclonal Antibody, unconjugated (bsm-4793M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEA (5F2) Monoclonal Antibody, Unconjugated (bsm-4793M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-4793M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-4793M-Cy3

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-4793M-Cy5

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-4793M-Cy7

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-4793M-FITC

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-4793M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-4793M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-1621m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-1621m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-1621m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-1621m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-1622m

Applications: WB, ELISA, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1622M-Cy5

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-1622M-Cy7

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-1622M-FITC

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-1622M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-1624M

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1624M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-1624M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-1624M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-1624M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-1624M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33048M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: Biotin

Catalog #1: bsm-33048M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: HRP

Catalog #1: bsm-1623M

Applications: ELISA, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1623M-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-1623M-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-1623M-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-1623M-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-1623M-Biotin

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-1623M-HRP

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Lane 1: Human Liver; Probed with Arginase-1 (2C12) Monoclonal Antibody (bsm-54331R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Arginase-1 (2C12) Monoclonal Antibody (bsm-54331R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54331R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated