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Formalin-fixed and paraffin embedded rat brain labeled with Anti-HSP70\/HSPA1A Polyclonal Antibody, Unconjugated (bs-0244R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with HSP70 1A\/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSP70 Antibody(bs-0244R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Testis lysates; Lane 2: Mouse Epididymis lysates; Lane 3: Mouse Seminal vesicle lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Testis lysates; Lane 7: Rat Spleen lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human A549 cell lysates; Lane 12: Human MCF-7 cell lysates probed with HSP70 1A\/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0244R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

HeLa cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Mouse Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with HSP70 (5G10) Monoclonal Antibody, Unconjugated (bsm-33048M) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bsm-33048M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Pig

Conjugate: Unconjugated

Catalog #1: bs-0244R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Biotin

Catalog #1: bs-0244R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: HRP

Catalog #1: bsm-33048M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: Biotin

Catalog #1: bsm-33048M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: HRP

Catalog #1: bs-0244R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy5

Catalog #1: bs-0244R-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy5.5

Catalog #1: bs-0244R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: PE

Catalog #1: bs-0244R-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy7

Catalog #1: bs-0244R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Cy3

Catalog #1: bs-0244R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: FITC

Catalog #1: bsm-33048M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: Cy3

Catalog #1: bsm-33048M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: Cy5

Catalog #1: bsm-33048M-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: Cy5.5

Catalog #1: bsm-33048M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: Cy7

Catalog #1: bsm-33048M-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: FITC

Catalog #1: bsm-33048M-PE

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: PE

Catalog #1: bsm-33048M-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33048M-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33048M-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33048M-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33048M-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33048M-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33048M-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33048M-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33048M-APC

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: APC

Catalog #1: bsm-33048M-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: APC-Cy5.5

Catalog #1: bsm-33048M-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Pig

Conjugate: APC-Cy7

Catalog #1: bsm-33048M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Pig

Conjugate: PE-Cy5

Catalog #1: bsm-33048M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Pig

Conjugate: PE-Cy5.5

Catalog #1: bsm-33048M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Pig

Conjugate: PE-Cy7

Catalog #1: bsm-33048M-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: PerCP

Catalog #1: bsm-33048M-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33048M-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Pig

Conjugate: RBITC