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IF (IHC-P) Antibodies



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Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-MAPKAPK2(Ser272)Polyclonal Antibody, Unconjugated (bs-5503R)at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-MAPKAPK2(Ser272)Polyclonal Antibody, Unconjugated (bs-5503R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Phospho-MAPKAPK2 (Ser272) ) Polyclonal Antibody, Unconjugated (bs-5503R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Phospho-MAPKAPK2 (Ser272) ) Polyclonal Antibody, Unconjugated (bs-5503R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5503r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human stomach carcinoma labeled withAnti-Folate Receptor 4 Polyclonal Antibody, Unconjugated (bs-13195R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Folate Receptor 4) Polyclonal Antibody, Unconjugated (bs-13195R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Folate Receptor 4) Polyclonal Antibody, Unconjugated (bs-13195R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-13195r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-10023P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (STAT4) Polyclonal Antibody, Unconjugated (bs-10023R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-10023r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7881R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1915R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11832R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti P63 protein\/P51A Polyclonal Antibody, Unconjugated (bs-0723R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: A431 Cell lysates; Probed with P63 Polyclonal Antibody, unconjugated (bs-0723R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0723R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FABP7\/BLBP\/FABP(brain)Polyclonal Antibody, Unconjugated (bs-0386R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates probed with Anti FABP7\/BLBP\/FABP(brain)Polyclonal Antibody, Unconjugated (bs-0386R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 15kD. Observed band size:15kD.\\n

Catalog #1: bs-0386R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Prostate tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (bs-5655R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (bs-5655R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (bs-5655R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-RAD9(Ser336) ) Polyclonal Antibody, Unconjugated (bs-5655R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5655R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-OCT1 Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-OCT1 Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (OCT-1 ) Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1075r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-1534R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

A549 cell lysates probed with Anti-kappa Opioid receptor Polyclonal Antibody, Unconjugated (bs-1094R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates probed with kappa Opioid receptor Polyclonal Antibody, Unconjugated (bs-1094R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1094R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H4 (tri methyl K20) Polyclonal Antibody, Unconjugated (bs-7403R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H4 (tri methyl K20)) Polyclonal Antibody, Unconjugated (bs-7403R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H4 (tri methyl K20)) Polyclonal Antibody, Unconjugated (bs-7403R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-7403R

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-10017P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates probed with Glycophorin A Polyclonal Antibody, Unconjugated (bs-11261R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10017R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0885R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-beta-Amyloid 1-40(CT) Polyclonal Antibody, Unconjugated (bs-0877R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U87MG cell lysates; Lane 2: Human SY5Y cell lysates; Lane 3: Human U251 cell lysates probed with beta-Amyloid 1-40 (CT) Polyclonal Antibody, Unconjugated (bs-0877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0877R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-0790R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti NF-L\/Neurofilament L\/Neurofilament 68 Polyclonal Antibody, Unconjugated (bs-0707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NF-L Polyclonal Antibody(bs-0707R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Human U251 cell lysates probed with NF-L Polyclonal Antibody, Unconjugated (bs-0707R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0707R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0587R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Smad4 Polyclonal Antibody, Unconjugated (bs-0585R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Cancer circulating cells\u00a0were obtained from patient whole blood, after AutoMAC depletion, CD45 negative cell were stained with EpCAM, MET, p16, Smad4 (Rabbit Anti-Smad4 Polyclonal Antibody, bs-0585R) and FxCycle etc, the imagery ware generated by Image Stream X \u00a0(Amnis). This image was generously provided by Peng Xu at the University of Chicago as part of the Bioss Discovery Program.\\n

Catalog #1: bs-0585R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney labeled with Anti-Thrombomodulin\/CD141 Polyclonal Antibody, Unconjugated (bs-0525R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 rat heart, L2 rat brain lysates probed (bs-0525R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 61kDa.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates ; Lane 5: Human Huvec cell lysates ; Lane 6: Human A549 cell lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-0525R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates ; Lane 5: Human Huvec cell lysates ; Lane 6: Human A549 cell lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-0525R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0525R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-Annexin V Polyclonal Antibody, Unconjugated (bs-0450R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human glioblastoma labeled with Rabbit Anti-Annexin V Polyclonal Antibody, Unconjugated (bs-0450R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Annexin V Polyclonal Antibody, Unconjugated (bs-0450R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Annexin V Polyclonal Antibody, Unconjugated ( bs-0450R ) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-0450R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse heart labeled with Anti AT2R\/AT1 Polyclonal Antibody, Unconjugated (bs-0438R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti AT2R\/AT1 Polyclonal Antibody, Unconjugated (bs-0438R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C. and DAPI staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AT2R) Polyclonal Antibody, Unconjugated (bs-0438R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0438R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with Leptin receptor\/Ob-R Polyclonal Antibody, Unconjugated (bs-0410R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Leptin receptor\/Ob-R Polyclonal Antibody(bs-0410R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0410R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human cervical carcinoma labeled with Anti-Runx3 Polyclonal Antibody (bs-0378R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse Lymph Node lysates probed with Runx3 Polyclonal Antibody, unconjugated (bs-0378R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0378R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Amylin Polyclonal Antibody, Unconjugated (bs-0057R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Amylin) Polyclonal Antibody, Unconjugated (bs-0057R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining

Catalog #1: bs-0057R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3128R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3036R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-Tau protein(Ser404)Polyclonal Antibody, Unconjugated (bs-2392R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: SH-SY5Y cell lysates; Lane 2: MCF-7 cell lysates probed with Tau protein(Ser579) Polyclonal Antibody, Unconjugated (bs-2392) Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2392R

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-2150R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-12456R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-ATM (Tyr170) Antibody(bs-11707R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-ATM (Ser794) Antibody(bs-11707R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-11707R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10376R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Antigen: bs-10007P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
A431 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with EGFR Antibody(bs-10007R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-10007R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-0992R

Applications: IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-0714R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Virus

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-EMR1 Polyclonal Antibody, Unconjugated (bs-7058R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
A549 cell lysates probed with EMR1 Polyclonal Antibody, Unconjugated (bs-7058R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U87MG cell lysates probed with EMR1 Polyclonal Antibody, Unconjugated (bs-7058R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
FFPE mouse liver labeled with Rabbit Anti-EMR1 Polyclonal Antibody (bs-7058R) at 1:200 followed by conjugation to the secondary antibody (Goat-Rabbit) and DAB staining.

Catalog #1: bs-7058r

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-1061R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3004R

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti- CCL11\/Eotaxin 1 Polyclonal Antibody (bs-1601R) at 1:100 for two hours at room temperature (green channel).
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CCL11\/Eotaxin 1 ) Polyclonal Antibody, Unconjugated (bs-1601R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1601R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPR92 Polyclonal Antibody, Unconjugated (bs-15366R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human Jurkat cell lysates probed with GPR92 Polyclonal Antibody, Unconjugated (bs-15366R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-15366R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-COX4\/COX IV-1 Polyoclonal Antibody, Unconjugated (bs-1533R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Raji cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A431 cell lysates probed with COX4I1 Polyclonal Antibody, Unconjugated (bs-1533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1533R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Jagged1\/CD339 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Jagged1) polyclonal Antibody, Unconjugated (bs-1448R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Recombinant human CD339 protein, N-His(bs-42225P) probed with Jagged1 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1448R

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti phospho-Androgen Receptor(Ser578) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Androgen Receptor(Ser578)) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0943R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0688r

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ABCG2\/CD338 Polyclonal Antibody, Unconjugated(bs-0662R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
L1 rat brain lysates L2 mouse embryo lysates probed with Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 72kD. Observed band size:72kD, 28kD.\\n

Catalog #1: bs-0662r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14473R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13999R

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated