phospho-AR/Androgen receptor (Ser578) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti phospho-Androgen Receptor(Ser578) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Androgen Receptor(Ser578)) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Androgen Receptor(Ser578)) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0943R
Product Name	phospho-AR/Androgen receptor (Ser578) Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt
Specificity	This phosphorylation site is homologous to that of Ser558 in Mouse and Ser561 in Rat.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser578 [CG(p-S)CK]
Modification Site	Ser578
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	367
Swiss Prot	P10275
Subcellular location	Cytoplasm, Nucleus
Synonyms	KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; AR
Background	Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Application Dilution
WB	IHC-P1:100-500
IHC-P	IFIHC-P1:100-500
IHC-F
IF
Flow-Cyt