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IF (IHC-P) Antibodies



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Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD

Catalog #1: bs-1032R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1083R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0699R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0947R-FITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Mouse splenocytes probed with Rabbit Anti-JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK2 Polyclonal Antibody(bs-0908R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human K562 cell lysates; Lane 2: Mouse BV-2 cell lysates probed with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0908R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0427R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (bs-23103R) at 1:400 overnight at 4\u00b0C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Ki-67 Polyclonal Antibody(bs-23103R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-23103R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Testis lysates; Lane 2: Mouse Testis lysates probed with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4597R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart lysates; Lane 2: Mouse Skeletal muscle lysates; Lane 3: Rat Heart lysates; Lane 4: Rat Skeletal muscle lysates; Lane 5: Human 293T cell lysates probed with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0611r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse lymph node lysate probed with CD8a Polyclonal Antibody (bs-10699R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse Spleen cells probed with CD8a Polyclonal Antibody, unconjugated (bs-10699R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Rat Thymus tissue lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-10699R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8 Polyclonal Antibody, Unconjugated (bs-10699R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-10699r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-9599R

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRE1a Polyclonal Antibody, Unconjugated (bs-8680R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Raji cell lysates; Lane 2: Human HeLa cell lysates probed with IRE1 Polyclonal Antibody, Unconjugated (bs-8680R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8680R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-8074R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-6640r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6216R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: RAW264.7 (Mouse) Cell Lysate probed with Alpha-synuclein(Ser129) Antibody, Unconjugated (bs-5628R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebellum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human U251 cell lysates; Lane 7: Human 293T cell lysates; Lane 8: Human Hela cell lysates probed with Alpha synuclein Polyclonal Antibody, Unconjugated (bs-5628R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SY5Y cell lysates; Lane 2: Human U87MG lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human HELA cell lysates; Lane 6: Human 293T cell lysates probed with phospho-Alpha synuclein (Ser129) Antibody, Unconjugated (bs-5628R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5628R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DDX58 (Thr170) Polyclonal Antibody, Unconjugated (bs-5300R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human MCF-7 cell lysates probed with phospho-DDX58 (Thr170) Polyclonal Antibody, Unconjugated (bs-5300R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5300R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-HMGCR Polyclonal Antibody, Unconjugated (bs-5068R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGCR Polyclonal Antibody, Unconjugated (bs-5068R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5068R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Thymus lysates probed with Perilipin A Polyclonal Antibody, Unconjugated (bs-3789R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Parotid gland lysates probed with Perilipin A Polyclonal Antibody, Unconjugated (bs-3789R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3789R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3851R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3794R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat uterus tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Estrogen receptor alpha (Ser118) Polyclonal Antibody, Unconjugated (bs-3130R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
MCF-7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nEstrogen Receptor alpha (S118) Polyclonal Antibody(bs-3130R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: MCF-7 cell lysates probed with Estrogen Receptor alpha (S118) Polyclonal Antibody, Unconjugated (bs-3130R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha (S118) Polyclonal Antibody, Unconjugated (bs-3130R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3130R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2578R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse brain lysates probed with Anti- Neuritin Polyclonal Antibody, Unconjugated (bs-2464R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U251 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Neuritin Polyclonal Antibody(bs-2464R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2464R

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2168R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-LPL protein Polyclonal Antibody, Unconjugated (bs-2336R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LPL protein\/Lipoprotein lipase Polyclonal Antibody, Unconjugated (bs-2336R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human HL-60 cell lysates probed with Lipoprotein lipase Polyclonal Antibody, Unconjugated (bs-2336R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2336R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded pig liver labeled with Anti-CK18 Polyclonal Antibody, Cy3 Conjugated (bs-2043R-Cy3) at 1:200 for 60 minutes at 37C.
PC12 cells probed with Rabbit Anti-CK18 Polyclonal Antibody (bs-2043R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Paraformaldehyde-fixed, paraffin embedded human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Rat Large intestine lysates probed with Cytokeratin 18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-2043R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung cancer labeled with Anti-CK7 (Human) Polyclonal Antibody, Unconjugated (bs-1610R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1610R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-Ubiquitin\/UBC\/UB Polyclonal Antibody, Unconjugated (bs-1549R) followed by conjugation to the secondary antibody and DAB staining
Ubiquitin protein probed with Rabbit Anti-Ubiquitin Polyclonal Antibody, Unconjugated (bs-1549R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Image provided by One World Lab validation program. PC3 cells probed with Ubiquitin Polyclonal Antibody (bs-1549R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Ubiquitin) polyclonal Antibody, Unconjugated (bs-1549R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1549R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded tumor tissue labeled with Anti-APOJ\/Clusterin-\u03b1\u03b2 Polyclonal Antibody, Unconjugated (bs-1354R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti APOJ\/CLUSTERIN Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD. Observed band size: 50kD
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-APOJ\/Clusterin Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Anti-APOJ\/Clusterin Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1354R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-1340R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Human 293T cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A549 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-1340R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FAK) polyclonal Antibody, Unconjugated (bs-1340R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (bs-1340R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1340R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Spinophilin\/Neurabin 2 Polyclonal Antibody, Unconjugated (bs-12146R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Neurabin 2 Polyclonal Antibody, Unconjugated (bs-12146R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Spinophilin\/Neurabin 2) Polyclonal Antibody, Unconjugated (bs-12146R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Spinophilin\/Neurabin 2) Polyclonal Antibody, Unconjugated (bs-12146R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-12146R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: HL60 cell lysates; Lane 2: Mouse testis lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates;Lane 2: Rat Cerebrum lysates;Lane 3: Mouse heart lysates; Lane 4: Rat heart lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RUNX1 + RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:400 overnight at 4\u00b0C, DAB staining.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RUNX1 + RUNX2 Polyclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1134R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10233r-fitc

Applications: WB, FCM, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2\/skp2 p45 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated(bs-1096R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Hela cells probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
293T lysates probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1096R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10341R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 1 Polyclonal Antibody (bs-0610R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse bone lysates probed with Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (bs-0610R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Heart tissue lysates probed with Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (bs-0610R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0610R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (bs-0520R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0520R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates and Human A549 cells probed with PACAP receptor 1 Polyclonal Antibody, unconjugated (bs-0198R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with PACAP receptor Polyclonal Antibody, Unconjugated (bs-0198R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0198R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (alpha 1 Antitrypsin) Polyclonal Antibody, Unconjugated (bs-0096R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining

Catalog #1: bs-0096R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP Polyclonal Antibody, Unconjugated (bs-0155R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0155R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human uterus labeled with Anti-Calretinin\/CA Polyclonal Antibody, Unconjugated (bs-0062R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Calretinin\/CA Polyclonal Antibody, Unconjugated (bs-0062R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates probed with Calretinin Polyclonal Antibody, Unconjugated (bs-0062R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0062R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysates probed with TAS1R3 Polyclonal Antibody, Unconjugated (bs-23618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human Jurkat cell lysates probed with TAS1R3 Polyclonal Antibody, Unconjugated (bs-23618R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23618R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with alpha smooth muscle Actin (2B10) Monoclonal Antibody (bsm-52396R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Flow cytometric analysis of Jurkat cells with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with ACTA2 (2B10) Monoclonal Antibody (bsm-52396R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52396r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Anti-ADFP Polyclonal Antibody, Unconjugated (bs-1164R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse liver lysates probed with ADFP Polyclonal Antibody, Unconjugated (bs-1164R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1164R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

This image was kindly submitted by Dr. Lajoie from the University of Wisconsin-Madison.\\n4% PFA fixed rat brain vascular endothelial cells (RBE4) labeled with RABBIT ANTI-ITM2A POLYCLONAL ANTIBODY, UNCONJUGATED (BS-9705R) 1:100 dilution, followed by conjugated secondary antibody and DAPI staining. Anti-ITM2A staining is shown in green with blue DAPI counterstain.
K562 cell lysates probed with ITM2A Polyclonal Antibody, Unconjugated (bs-9705R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9705R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9505r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MARCH5) Polyclonal Antibody, Unconjugated (bs-9339R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MARCH5) Polyclonal Antibody, Unconjugated (bs-9339R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-9339R

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated