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IF (IHC-P) Antibodies



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Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-Galectin-9 Polyclonal Antibody (bs-0604R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 9 Polyclonal Antibody, Unconjugated (bs-0604R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 9 Polyclonal Antibody, Unconjugated (bs-0604R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 9 Polyclonal Antibody, Unconjugated (bs-0604R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0604R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PAK7\/PAK5\/MBT Polyclonal Antibody, Unconjugated (bs-0655R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti PAK7\/PAK5\/MBT Polyclonal Antibody, Unconjugated (bs-0655R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAK5 Polyclonal Antibody, Unconjugated (bs-0655R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAK5 Polyclonal Antibody, Unconjugated (bs-0655R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0655R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

paraformaldehyde-fixed, paraffin embedded mouse placenta tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated (bs-0567R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
paraformaldehyde-fixed, paraffin embedded mouse placenta tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated (bs-0567R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
paraformaldehyde-fixed, paraffin embedded mouse placenta tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated (bs-0567R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-0567R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ILK-1 Polyclonal Antibody (bs-0317R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 human colon carcinoma lysates probed with Anti ILK-1 Polyclonal Antibody, Unconjugated (bs-0317R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 50kD. Observed band size:50kD.\\n
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-ILK-1 Polyclonal Antibody (bs-0317R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-ILK-1 Polyclonal Antibody (bs-0317R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n

Catalog #1: bs-0317R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0505R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse PBMC cell lysates; Lane 2: Mouse Thymus lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Thymus lysates probed with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10211r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 rat heart lysates, L2 rat brain lysates probed: Anti-Tubulin-alpha (bs-0159R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 55kDa
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebellum lysates; Lane 4: Mouse Cerebellum lysates probed with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human 293T cell lysates probed with Tubulin-alpha (Loading Control) Polyclonal Antibody, Unconjugated (bs-0159R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U-2 OS cell lysates; Lane 5: Human A431 cell lysates probed with Tubulin-alpha (Loading Control) Polyclonal Antibody, Unconjugated (bs-0159R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0159r

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20443R) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20443R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Cerebral Cortex; Lane 2: Rat Hippocampus; 40ug loaded in each lane; Probed with Tau Polyclonal Antibody, unconjugated (bs-20443R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum; Lane 2: Mouse Hippocampus; 40ug loaded in each lane; Probed with Tau Polyclonal Antibody, unconjugated (bs-20443R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-20443R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and rat lung tissue labeled with Anti-B7-H1\/PD-L1\/CD274 Polyclonal Antibody, Unconjugated(bs-1103R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with CD274 Polyclonal Antibody, unconjugated (bs-1103R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD274 Polyclonal Antibody(bs-1103R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1103R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-9925P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CACNA1S Polyclonal Antibody, Unconjugated (bs-9925R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CACNA1S Polyclonal Antibody, Unconjugated (bs-9925R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-9925R

Applications: ELISA, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8592R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-8870R

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8612R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti CEP55 Polyclonal Antibody, Unconjugated (bs-7742R) followed by conjugation to the secondary antibody and DAB staining
Mouse testis lysates probed with CEP55 Polyclonal Antibody, Unconjugated (bs-7742R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7742R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-7544R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse testicular lysate probed with Rabbit Anti-MTSS1 Polyclonal Antibody (bs-6075R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Lane 1: Human HUVEC cell lysates; Lane 2: Human U87MG cell lysates probed with MTSS1 Polyclonal Antibody, Unconjugated (bs-6075R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6075R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: rat brain lysates Lane 2: rat brain lysates probed with Anti Pancreatic Amylase Polyclonal Antibody, Unconjugated (bs-4030R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 57kD. Observed band size: 57kD.
Lane 1: A431 cell lysates probed with Pancreatic Amylase Polyclonal Antibody, Unconjugated (bs-4030) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4030R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH\/3T3 cell lysates probed with YAP1(Tyr407) Polyclonal Antibody, Unconjugated (bs-3476R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YAP1(Tyr407) Polyclonal Antibody, Unconjugated (bs-3476R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YAP1(Tyr407) Polyclonal Antibody, Unconjugated (bs-3476R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Lung lysates; Lane 2: Rat Lung lysates probed with YAP1(Tyr407) Polyclonal Antibody, Unconjugated (bs-3476R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3476R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-BTK (Tyr223) Polyclonal Antibody, Unconjugated (bs-3069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-BTK (Tyr223) Polyclonal Antibody, Unconjugated (bs-3069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3069R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Antigen: bs-2680P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Mouse splenocytes probed with Rabbit Anti-NFAM1 Polyclonal Antibody, Unconjugated (bs-2680R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).
U937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFAM1 Polyclonal Antibody(bs-2680R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2680R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver labeled with Anti-P450 1A2\/CYP1A2 Polyclonal Antibody, Unconjugated (bs-2589R) followed by conjugation to the secondary antibody and DAPI staining
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCytochrome P450 1A2 Polyclonal Antibody(bs-2589R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: HepG2 cell lysates probed with P450 1A2 Polyclonal Antibody, Unconjugated (bs-2589R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2589R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2477R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2133R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat testis lysates probed with Rabbit Anti-GSK-3 Beta(Ser9) Polyclonal Antibody, Unconjugated (bs-2066R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Tissue\/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser9)) polyclonal Antibody, Unconjugated (bs-2066R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2066R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-15513R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 Rat brain lysate L2 human colon carcinoma 30ug, probed (bs-1548R) at 1:200 in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted and observed band size: 74kDa.
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nFOXO3A Polyclonal Antibody(bs-1548R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human DU145 cell lysates probed with FOXO3A Polyclonal Antibody, Unconjugated (bs-1548R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1548R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-CD13\/APN\/ANPEN Polyclonal Antibody, Unconjugated (bs-1383R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-CD13\/APN\/ANPEN Polyclonal Antibody, Unconjugated (bs-1383R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 probed with CD13\/APN\/ANPEN Polyclonal Antibody (bs-1383R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
U-937 (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CELF3 Antibody (bs-10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1383R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain lysates, L2 rat heart lysates probed with Anti-Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 42kD

Catalog #1: bs-1365R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-FHOD1 Polyclonal Antibody, Unconjugated (bs-13158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with FHOD1 Polyclonal Antibody, Unconjugated (bs-13158R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1:Mouse muscle lysates; Lane 2:Rat Cerebrum lysates probed with FHOD1 Polyclonal Antibody, Unconjugated (bs-13158R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-13158R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1286R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1299r

Applications: WB, ELISA, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SNAP25 Polyclonal Antibody, Unconjugated (bs-1131R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-SNAP25 Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FiTC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti SNAP25 Polyclonal Antibody, Unconjugated (bs-1131R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 23kD. Observed band size: 23kD.

Catalog #1: bs-1131R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-QRFP Polyclonal Antibody, Unconjugated (bs-11534R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
paraformaldehyde-fixed, paraffin embedded human neurofibroma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-QRFP Polyclonal Antibody, Unconjugated (bs-11534R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with QRFP Polyclonal Antibody, Unconjugated (bs-11534R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11534R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Anti- ATP1b2\/Na+K+ATPase Polyclonal Antibody, Unconjugated (bs-1152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 rat colon lysates probed with Anti ATP1b2\/Na+K+ATPase Polyclonal Antibody, Unconjugated (bs-1152R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 33kD. Observed band size: 33kD.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Human K562 cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with ATPase Na+\/ K+ beta 2 Polyclonal Antibody, Unconjugated (bs-1152R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1152r

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (bs-10414R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat embryo labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (bs-10414R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Anti-Nanog Polyclonal Antibody, Unconjugated (bs-10414R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A431 lysates probed with Nanog Polyclonal Antibody, Unconjugated (bs-10414R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-10414R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10006R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-C-Jun Polyclonal Antibody (bs-0670R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-Jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-0670R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysates probed with Anti-APOA1 Polyclonal Antibody, Unconjugated (bs-0849R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-APOA1 Polyclonal Antibody, Unconjugated (bs-0849R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.

Catalog #1: bs-0849R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (bs-0721R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0721R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Human HL-60 lysates probed with Rabbit Anti-CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0569R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed human oral squamous cell carcinoma labeled with Anti-Survivin Polyclonal Antibody, Cy3 conjugated (bs-0615R-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C, followed by DAPI staining
Human Gastric Cancer Cells labeled with Anti-Survivin Polyclonal Antibody, Unconjugated (bs-0615R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Survivin Polyclonal Antibody, Unconjugated (bs-0615R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Rabbit Anti-Survivin Polyclonal Antibody, Unconjugated (bs-0615R) at 1:200 followed by Goat Anti-Rabbit IgG, FITC conjugated (bs-0295G-FITC) used at 1:300 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0615R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung tissue labeled with Rabbit Anti-TSHR Polyclonal Antibody (bs-0206R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse thymocytes probed with Rabbit Anti-TSHR Polyclonal Antibody, Unconjugated (bs-0206R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).
Lane 1: Human Jurkat cell lysates; Lane 2: Human TT cell lysates probed with TSHR (CT) Polyclonal Antibody, Unconjugated (bs-0206R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0206r

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-FGF1 Polyclonal Antibody, Unconjugated (bs-0229R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FGF1 Polyclonal Antibody(bs-0229R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0229R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 8 + 18) Monoclonal Antibody, Unconjugated (bsm-52419R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 8 + 18) Monoclonal Antibody, Unconjugated (bsm-52419R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 8 + 18) Monoclonal Antibody, Unconjugated (bsm-52419R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 8 + 18) Monoclonal Antibody, Unconjugated (bsm-52419R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-52419r

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33400M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ASC Polyclonal Antibody, Unconjugated (bs-6741R) at 1:800 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ASC Polyclonal Antibody, Unconjugated (bs-6741R) at 1:800 overnight at 4\u00b0C, DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASC Polyclonal Antibody(bs-6741R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASC Polyclonal Antibody(bs-6741R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6741R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13385R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Heart lysates; Lane 2: Mouse Skin lysates; Lane 3: Mouse Thyroid gland lysates probed with NGFB Polyclonal Antibody, Unconjugated (bs-10806R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysates; Lane 3: Human U-87 MG cell Lysates; Lane 4: Human SH-SY5Y cell Lysates; Lane 5: Human U-2 OS cell Lysates. Probed with NGFB\u00a0polyclonal Antibody, unconjugated (bs-10806R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (bs-10806R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10806R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated