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IF (IHC-P) Antibodies



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Catalog #1: bs-0023M-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-2022M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Virus

Conjugate: Cy5.5

Paraformaldehyde-fixed, paraffin-embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4\u00b0C, followed by DAB staining.
Paraformaldehyde-fixed, paraffin-embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4\u00b0C, followed by DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Gamma Tubulin Monoclonal Antibody(bsm-33313M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with Gamma Tubulin monoclonal antibody, unconjugated (bsm-33313M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33313m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33279M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-33279M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-33279M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-33279M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-10895m-fitc

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-10895m-cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-10895m-cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-10895m-cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-1623m-fitc

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-1623m-cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-1623m-cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-1623m-cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-1623m

Applications: ELISA, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33093M-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33093M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33093M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33093M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Lane 1: Hela lysates; Lane 2: NIH3T3 lysates probed with Histone H3 (di methyl K79) Polyclonal Antibody, Unconjugated (bs-33093M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (di methyl K79) Monoclonal Antibody, Unconjugated (bsm-33093M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (di methyl K79) Monoclonal Antibody, Unconjugated (bsm-33093M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (di methyl K79) Monoclonal Antibody, Unconjugated (bsm-33093M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33093M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33034m-fitc

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33034m-cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33034m-cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33143m-cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33143m-cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33143m-cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33323m-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33323m-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33323m-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Lane 1: 293; Lane 2: F9; Lane 3: PC12; Lane 4: Hela lysates probed with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human uterus \r\ntissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin A2 (2E9) Monoclonal Antibody (bsm-52043M) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52043m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cell lysates probed with PNSE (15E2) Monoclonal Antibody, unconjugated (bsm-33072M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
U87MG cell lysates probed with PNSE (15E2) Monoclonal Antibody, unconjugated (bsm-33072M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NSE (15E2) Monoclonal Antibody, Unconjugated (bsm-33072M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NSE (15E2) Monoclonal Antibody, Unconjugated (bsm-33072M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33072M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33187m-cy7

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m-cy5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33187m-cy3

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33035m-fitc

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33035m-cy7

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33035m-cy5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33035m-cy3

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33293M-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bsm-33293M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bsm-33293M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bsm-33293M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bsm-33219M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy7

Catalog #1: bsm-33219M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5

Catalog #1: bsm-33219M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy3

Catalog #1: bsm-33042m-fitc

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33042m-cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7