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IF (IHC-P) Antibodies



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Formalin-fixed and paraffin embedded human kidney tissue labeled with Anti-Acetyl-Histone H1b(K53) Polyclonal Antibody (bs-0931R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spleen tissue labeled with Anti-Acetyl-Histone H1b(K53) Polyclonal Antibody (bs-0931R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H1 (Lys52) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H1 (Lys52) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0931R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1:Huh7 lysates Lane 2: A549 lysates probed with Rabbit Anti-Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: NIH\/3T3 cell lysates; Lane 2:Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: U2os cell lysates probed with Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3776R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human ovarian cancer tissue labeled with Rabbit Anti-Acetyl-p53(K382) Polyclonal Antibody (bs-0905R), Unconjugated followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse brain lysates Lane 2: rat kidney lysates probed with Anti Acetyl-p53(K382) Polyclonal Antibody, Unconjugated (bs-0905R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 53kD. Observed band size: 53kD.
Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl p53(K382) Polyclonal Antibody, Unconjugated (bs-0905R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nAcetyl p53(K382) Polyclonal Antibody, Unconjugated (bs-0905R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0905R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: SiHa Cells; Probed with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54330r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen labeled with Anti Acetyl-Histone H3 Polyclonal Antibody, Unconjugated (bs-3774R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl Histone H3(K23) Polyclonal Antibody, Unconjugated (bs-3774R) at 1:400 overnight at 4\u00b0C, DAB staining.
Hela cells were fixed with 70% ice-cold methanol overnight at 4\u2103\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Acetyl Histone H3(K23)Antibody(bs-3774R) at 1\uff1a50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl Histone H3(K23) Polyclonal Antibody, Unconjugated (bs-3774R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3774R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Piotr Mamczur as part of the free sample program. Immunofluorescent localization of acetylated histone H4 in mouse squamous cell cancer (KLN-205 cell line) with BS-4707R (1:20) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Histone H4 (Acetyl K16) Antibody(bs-4707R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-4707R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3746R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-10721R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: RBITC

Catalog #1: bs-10721R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-10721R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bs-10721R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy7

Catalog #1: bs-10721R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-10721R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bs-10721R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-10721R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-10721R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-10721R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-10721R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-10721R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-10721R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-10721R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-4707R-RBITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-4707R-PerCP-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-4707R-PerCP

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-4707R-APC-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-4707R-APC-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-4707R-APC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: APC

Catalog #1: bs-4707R-BF750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-4707R-BF680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-4707R-BF647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-4707R-BF594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-4707R-BF555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-4707R-BF488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-4707R-BF405

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-4707R-BF350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-4016R-RBITC

Applications: WB, IF

Reactivity: Rat

Conjugate: RBITC

Catalog #1: bs-4016R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-4016R-PerCP

Applications: WB, IF

Reactivity: Rat

Conjugate: PerCP

Catalog #1: bs-4016R-APC-Cy7

Applications: WB

Reactivity: Rat

Conjugate: APC-Cy7

Catalog #1: bs-4016R-APC-Cy5.5

Applications: WB, IF

Reactivity: Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-4016R-APC

Applications: WB, IF

Reactivity: Rat

Conjugate: APC

Catalog #1: bs-4016R-BF750

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-4016R-BF680

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-4016R-BF647

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-4016R-BF594

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-4016R-BF555

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-4016R-BF488

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-4016R-BF405

Applications: WB, IF

Reactivity: Rat

Conjugate: AbBy Fluor-405