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IF (IHC-P) Antibodies



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Catalog #1: bs-0379R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Heart lysates probed with Anti-EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:300 in dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates; Lane 2: Mouse Cerebrum cell lysates; Lane 3:Rat Heart cell lysates; Lane 4: Mouse Heart cell lysates probed with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7541R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-TFF2 Polyclonal Antibody, Unconjugated (bs-1921R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-TFF2 Polyclonal Antibody, Unconjugated (bs-1921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Small intestine tissue lysates; Lane 2: Mouse Pancreas tissue lysates probed with TFF2 Polyclonal Antibody, Unconjugated (bs-1921R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1921R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD20 Polyclonal Antibody (bs-0080R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human larynocarcinoma labeled with Anti-CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Bone lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0080r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD134 Polyclonal Antibody, Unconjugated (bs-2685R) followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2685R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1\/CHIP Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 28kD. Observed band size: 28kD.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
HeLa cells were stained with AQP1 Polyclonal Antibody, Unconjugated(bs-1506R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Human 293T cells probed with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).\\n

Catalog #1: bs-1506R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human spleen labeled with Rabbit Anti MCSF\/M-CSF Polyclonal Antibody, Unconjugated (bs-4910R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysate probed with Rabbit Anti-MCSF Polyclonal Antibody (bs-4910R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
A549 cells probed with Rabbit Anti-MCSF Polyclonal Antibody (bs-4910R) at 1:50 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).

Catalog #1: bs-4910R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TGF Beta 1+2+3 Polyclonal Antibody, Unconjugated (bs-4538) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rabbit spleen lysates probed with TGF beta 1+2+3 Polyclonal Antibody, Unconjugated (bs-4538R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
MDA-MB-231 lysates probed with TGF Beta 1+2+3 Polyclonal Antibody, unconjugated (bs-4538R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Mouse Stomach lysates probed with TGF Beta 1+2+3 Polyclonal Antibody, unconjugated (bs-4538R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-4538R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti NOX2\/gp91phox Polyclonal Antibody, Unconjugated (bs-3889R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-NOX2\/gp91phox Polyclonal Antibody, Unconjugated (bs-3889R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse spleen lysates probed with NOX2\/gp91phox Polyclonal Antibody, unconjugated (bs-3889R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Lane 1: Rat Bronchus tissue lysates; Lane 2: Rat Esophagus tissue lysates; Lane 3: Human HL60 cell lysates probed with NOX2 Polyclonal Antibody, Unconjugated (bs-3889R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3889R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2549R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled Anti-HPV16 E6 + HPV18 E62 Polyclonal Antibody, Unconjugated (bs-1719R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-1719P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: HPV16 E6 + HPV18 E6 fusion protein (bs-49102P) 0.1ug Lysates; Lane 2: HPV16 E6 + HPV18 E6 fusion protein (bs-49102P) 0.05ug Lysates. Probed with HPV16 E6 + HPV18 E6 polyclonal Antibody, unconjugated (bs-1719R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HPV16 E6 + HPV18 E6 Polyclonal Antibody(bs-1719R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1719R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Mouse Kidney lysates probed with Rabbit Anti-ENPP1 Polyclonal Antibody, Unconjugated (bs-1760R) at 1:300 overnight at 4˚C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Huvec cell lysates probed with ENPP1 Polyclonal Antibody, Unconjugated (bs-1760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1760R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CYP19\/CYP19A1 Polyclonal Antibody, Unconjugated (bs-1292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CYP19\/CYP19A1 Polyclonal Antibody, Unconjugated (bs-1292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with Aromatase Polyclonal Antibody, Unconjugated (bs-1292R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1292R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-IL-12 Polyclonal Antibody, Unconjugated (bs-0767R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Lane 2: Mouse Spleen lysates; Lane 3: NIH'3T3; Lane 4: RAW246.7 lysates probed with IL12 Polyclonal Antibody, Unconjugated (bs-0767R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Blood cell lysates; Lane 3: Mouse RAW264.7 cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Rat Spleen lysates; Lane 6: Rat Liver lysates probed with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0767R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0862R

Applications: WB, ELISA, IHC-P, IF(IHC-P), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0586R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated(bs-0349R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0349R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat\r\n placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rhuman\r\n placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0117r

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Lane 1: mouse brain lysates probed with Rabbit Anti-LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 followed by conjugation to the secondary antibody (bs-0295G-HRP) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-8878R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-CIDEC Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-NFAT4 Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse stomach wall labeled with Anti-CIDEC Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CIDEC Polyclonal Antibody (bs-6796R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-6796R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung labeled with Rabbit Anti-Complement fragment 3c Polyclonal Antibody (bs-6416R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Complement C3 Polyclonal Antibody, Unconjugated (bs-6416R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Complement C3 Polyclonal Antibody, Unconjugated (bs-6416R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-6416r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2636R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human cervical carcinoma labeled with Rabbit Anti-CD160\/By55 Polyclonal Antibody ( bs-2526R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD160\/BY55 Polyclonal Antibody (bs-2526R), Unconjugated at 1:200 followed by conjugation to the secondary antibody
L1 rat brain, L2 rat kidney lysates probed (bs-2526R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 27kDa.

Catalog #1: bs-2526R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Mouse hippocampus labeled with Anti- Ntn1, Unconjugated (bs-1858R) followed by conjugation to the secondary antibody
Mouse heart lysates probed with Rabbit Anti-Netrin 1 Polyclonal Antibody, Unconjugated (bs-1858R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Netrin 1 Polyclonal Antibody, Unconjugated (bs-1858R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Netrin 1 Polyclonal Antibody, Unconjugated (bs-1858R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-1858R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tRabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated ( \\tbs-13239R ) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy)and used at 1:200 dilution for 40 minutes at 37\u00b0CDAPI(5ug\/ml) was used to stain the cell nuclei

Catalog #1: bs-13239r

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-10181R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: Unconjugated

U251 lysates probed with Rabbit Anti-DRD2 Polyclonal Antibody, Unconjugated (bs-1008R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DRD2 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-1008R) at 1:400 overnight at 4\u00b0C, followed by an SP Kit (Rabbit) and DAB staining.
Lane 1: Mouse BV-2 cell lysates; Lane 2: Rat RSC96 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human U-87MG cell lysates probed with DRD2 Polyclonal Antibody, Unconjugated (bs-1008R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1008R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-TGF Beta R1\/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 mouse embryo lysates probed with Anti TGF Beta R1\/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 55kD. Observed band size:55kD.\\n
Lane 1: Mouse Placenta lysates; Lane 2: Rat Small intestine lysates probed with TGF Beta R1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0638R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10031R

Applications: WB, ELISA, IHC-F, IF(IHC-P)

Reactivity: Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Insulin Polyclonal Antibody, Unconjugated(bs-0056R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-Insulin Polyclonal Antibody, Unconjugated(bs-0056R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse Pancreas (C57BL\/6 strain) fixed in 4% PFA for 2 hours at RT, then transferred to 30% sucrose overnight at 4\u00b0C before embedding in OCT mounting medium. Treated with TBS containing 5% goat serum and 0.3% Triton X-100, to block unspecific binding sites and permeabilize cell membranes, for 30min at RT. Antibody (bs-0056R-FITC) diluted at 1:100 in TBS containing 2% goat serum applied and incubated overnight at 4\u00b0C. Rinse sections in TBS 2x5min on a shaker, mount section with vectorshield (with DAPI), then cover them with coverslips and seal the edge of the coverslip on the slide with nail polish. This image was kindly submitted by an end-user from the National Children's Research Centre, Our Lady's Children's Hospital, Dublin, Ireland.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Insulin Polyclonal Antibody, Unconjugated (bs-0056R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0056R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Tyrosine Hydroxylase(Neuronal Marker)\/Tyk2\/TYH Polyclonal Antibody, Unconjugated (bs-0016R) 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
RSC96 probed with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 6ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human PC-3 lysates probed with Tyrosine Hydroxylase Polyclonal Antibody, unconjugated (bs-0016R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0016R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-8662P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Anti-BASP1 Polyclonal Antibody, Unconjugated (bs-8662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-8662R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colitis tissue labeled with IL-15 Polyclonal Antibody, Unconjugated (bs-1829R) at 1:200 dilution, followed by conjugation to the secondary antibody and DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IL-15) polyclonal Antibody, Unconjugated (bs-1829R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1829R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon tissue labeled with Anti-Metallothionein Polyclonal Antibody, Unconjugated (bs-1328R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Metallothionein Polyclonal Antibody, Unconjugated (bs-1328R) at 1:200 overnight at 4\u00b0C, DAB staining.
U937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nMetallothionein Polyclonal Antibody(bs-1328R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1328R

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0672R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8585R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse small intestine labeled with Anti SLCO2B1 Polyclonal Antibody, Unconjugated (bs-3913R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SLCO2B1\/OATPB Polyclonal Antibody, Unconjugated (bs-3913R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1:Mouse cerebrum lysates; Lane 2:Rat cerebrum lysates probed with SLCO2B1 Polyclonal Antibody, Unconjugated (bs-3913R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3913R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated (bs-3195R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3195R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse lung lysates probed with Anti-IL-1R2 Polyclonal Antibody (bs-2595R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.\\n
RSC96 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nIL-1R2 Antibody\uff08bs-2595R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Lane 1: SW480 cell lysates probed with IL-1R2 Polyclonal Antibody, Unconjugated (bs-2595R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates probed with 1R2 Polyclonal Antibody, Unconjugated (bs-2595R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2595R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Antigen: bs-2033P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: E.coli lysates; Lane 2: E.coli lysates; Probed with E.coli DH-5 Alpha Polyclonal Antibody, Unconjugated (bs-2033R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2033R

Applications: WB, ELISA, IF(IHC-P)

Reactivity: Bacteria, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-UCP-1 Polyclonal Antibody, Unconjugated (bs-1925R) at 1:300 followed by conjugation to the secondary antibody and DAPI staining
Lane 1: Mouse Thymus lysates probed with UCP-1 Polyclonal Antibody, Unconjugated (bs-1925R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1925R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse placenta lysates probed with AP-TNAP\/ALPL Polyclonal Antibody, unconjugated (bs-1535R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
HepG2 cells probed with AP-TNAP\/ALPL Polyclonal Antibody, unconjugated (bs-1535R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

Catalog #1: bs-1535R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

HeLa cells probed with Rabbit Anti-VEGFR2 Polyclonal Antibody (bs-10412R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
Formalin-fixed and paraffin embedded mouse placenta tissue labeled with Anti-VEGFR2 Polyclonal Antibody, Unconjugated (bs-10412R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody \uff08bs-10412R\uff09 for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bs-10412r

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung cancer labeled with Rabbit Anti-BDKRB1 Polyclonal Antibody, Unconjugated (bs-8675R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver tissue lysates; Lane 2: Human SiHa cell lysates; Lane 3: Human LOVO cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human U251 cell lysates probed with BDKRB1 Polyclonal Antibody, Unconjugated (bs-8675R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8675R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti PAPPA Polyclonal Antibody, Unconjugated (bs-6618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-6618P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Catalog #1: bs-6618R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody (bs-4611R) at 1:50 for 40 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) FITC Conjugated Secondary Antibody.
Image provided the Independent Validation Program, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Image provided by Independent Validation program, badge number 029457:Formalin-fixed and paraffin embedded human breast labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated (bs-4611R) at 1:200 overnight at 37\u00b0C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) at 1:200, 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue,C-0033) was used to stain the cell nuclei.\\n

Catalog #1: bs-4611R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54\/ICAM-1 Polyclonal Antibody (bs-0608R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54\/ICAM-1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 60kD. Observed band size: 60kD.
Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Lane 1: Hela cell lysates; Lane 2: Hela KO \r\nICAM1 cell lysates probed with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0608R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6795R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated