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P2Y8 Polyclonal Antibody

Raji lysates probed with P2Y8 Polyclonal Antibody, Unconjugated (bs-12073R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raji lysates probed with P2Y8 Polyclonal Antibody, Unconjugated (bs-12073R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raji cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P2Y8 Polyclonal Antibody(bs-12073R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raji cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P2Y8 Polyclonal Antibody(bs-12073R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-12073R
Product Name P2Y8 Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human P2Y8
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 286530
Swiss Prot Q86VZ1
Subcellular location Cell membrane
Synonyms P2RY8; P2Y receptor family member 8; P2RY8_HUMAN; P2Y purinoceptor 8; P2Y8; Purinergic receptor P2Y G protein coupled 8.
Background G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G-protein coupled receptors translate extracellular signals into intracellular signals (G-protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. P2RY8 (purinergic receptor P2Y, G-protein coupled, 8), also known as P2Y8, is a 359 amino acid multi-pass membrane protein that localizes to the cell membrane and belongs to the G protein-coupled receptor family. Expressed at low levels in lung, heart and kidney, P2RY8 may function as a receptor for purines that are coupled to G proteins and may also play a role in mental retardation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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