DDX19A Polyclonal Antibody

$Mouse heart lysates probed with Rabbit Anti-DDX19A\/B Polyclonal Antibody, Unconjugated (bs-14717R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.$

Mouse heart lysates probed with Rabbit Anti-DDX19A\/B Polyclonal Antibody, Unconjugated (bs-14717R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.

$Lane 1: Hela cell lysates probed with DDX19A\/B Polyclonal Antibody, Unconjugated (bs-14717R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Hela cell lysates probed with DDX19A\/B Polyclonal Antibody, Unconjugated (bs-14717R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Spleen lysates probed with DDX19A\/B Polyclonal Antibody, Unconjugated (bs-14717R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Spleen lysates probed with DDX19A\/B Polyclonal Antibody, Unconjugated (bs-14717R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Hamster

Overview
Catalog #	bs-14717R
Product Name	DDX19A Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody may cross react with both DDX19A and DDX19B due to sequence similarity.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Hamster
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DDX19A
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	11269, 55308
Swiss Prot	Q9UMR2, Q9NUU7
Subcellular location	Cytoplasm, Nucleus
Synonyms	ATP-dependent RNA helicase DDX19B; DDX19B; DEAD box RNA helicase DEAD5 DEAD box protein 19B; DBP5, DDX19, TDBP; ATP-dependent RNA helicase DDX19A; DDX19-like protein; DEAD box protein 19A; DDX19A; DDX19L
Background	ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200