MASP2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat ileum labeled with Anti- MASP2 Polyclonal Antibody, Unconjugated (bs-1980R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat liver labeled with Anti- MASP2 Polyclonal Antibody, Unconjugated (bs-1980R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Lane 1: mouse lung lysates Lane 2: mouse brain lysates probed with Rabbit Anti-MASP2 Polyclonal Antibody, Unconjugated (bs-1980R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 74kD. Observed band size: 74kD.

$Paraformaldehyde-fixed, paraffin embedded; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MASP2 Polyclonal Antibody, Unconjugated (bs-1980R)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MASP2 Polyclonal Antibody, Unconjugated (bs-1980R)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Horse

Overview
Catalog #	bs-1980R
Product Name	MASP2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MASP2
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10747
Swiss Prot	O00187
Subcellular location	Secreted
Synonyms	sMAP; MAP19; MASP-2; MASP1P1; Mannan-binding lectin serine protease 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP2
Background	Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200