Phospho-CEBP alpha (Thr222 + Thr226) Polyclonal Antibody

$Antigen: bs-3061P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-3061P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

$Lane 1: mouse mouse lysates Lane 2: mouse heart lysates probed with Anti Phospho-CEBP alpha (Thr222\/226)Polyclonal Antibody, Unconjugated (bs-3061R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 45kD. Observed band size: 45kD.$

Lane 1: mouse mouse lysates Lane 2: mouse heart lysates probed with Anti Phospho-CEBP alpha (Thr222\/226)Polyclonal Antibody, Unconjugated (bs-3061R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 45kD. Observed band size: 45kD.

$Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Phospho-CEBP alpha (Thr222 + Thr226) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Phospho-CEBP alpha (Thr222 + Thr226) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-3061R
Product Name	Phospho-CEBP alpha (Thr222 + Thr226) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This phosphorylated site is homologous to that of Thr222 in Mouse and Rat.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human CEBP alpha around the phosphorylation site of Thr226
Modification Site	Thr226
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1050
Swiss Prot	P49715
Subcellular location	Nucleus
Synonyms	CEBP; C/EBP-alpha; CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA
Background	C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200