phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, FITC Conjugated

Molt-4 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) antibody, FITC Conjugated\uff08bs-3332R-FITC) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).