PHLP Polyclonal Antibody

$Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Kidney tissue lysates; Lane 7: Human HUVEC cell lysates; Lane 8: Human K562 cell lysates probed with PHLP Polyclonal Antibody, Unconjugated (bs-5812R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Kidney tissue lysates; Lane 7: Human HUVEC cell lysates; Lane 8: Human K562 cell lysates probed with PHLP Polyclonal Antibody, Unconjugated (bs-5812R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-5812R
Product Name	PHLP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PHLP
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5082
Swiss Prot	Q13371
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	PhLP; Phosducin-like protein; PDCL; PHLOP1; PhLP1
Background	Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Isoform 2: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200