BRD1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-BRD1 Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-BRD1 Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BRD1 Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BRD1 Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Mouse Testis lysates probed with BRD1 Polyclonal Antibody, Unconjugated (bs-8426R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-8426R
Product Name	BRD1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BRD1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23774
Swiss Prot	O95696
Subcellular location	Nucleus
Synonyms	BRL; BRPF1; BRPF2; Bromodomain-containing protein 1; BR140-like protein; Bromodomain and PHD finger-containing protein 2; BRD1
Background	Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200