MBP Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with MBP monoclonal antibody, unconjugated (bsm-33932M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MBP monoclonal antibody, unconjugated (bsm-33932M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue).\r\nDAPI (dark blue) was used as a nuclear counter stain.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue).\r\nDAPI (dark blue) was used as a nuclear counter stain.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue).\r\nDAPI (dark blue) was used as a nuclear counter stain.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue).\r\nDAPI (dark blue) was used as a nuclear counter stain.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue).\r\nDAPI (dark blue) was used as a nuclear counter stain.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue).\r\nDAPI (dark blue) was used as a nuclear counter stain.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33932m-100ul
Product Name	MBP Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human MBP
Clonality	Monoclonal
Clone #	7B12
Isotype	IgG1, k
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4155
Swiss Prot	P02686
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Myelin Basic Protein; Myelin basic protien; GDB; Golli MBP; Hemopoietic MBP; HMBPR; HUGO; MBP; MGC99675; MLD; Myelin A1 Protein; Myelin Deficient; Myelin Membrane Encephalitogenic Protein; SHI; Shiverer; SP; MBP_HUMAN
Application Dilution
WB	1:1000-2000
IHC-P	IHC-P1:200-1000
IHC-F	IHC-F1:200-1000
IF(IHC-P)	IFIHC-P1:200-1000