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Human Vimentin Ready-To-Use IHC Kit

Human Vimentin Ready-To-Use IHC Kit

Cat. No.: IHC0113H
Sample Type:
FFPE tissue
Size:
50T (including a control slide)
Storage and Stability:
Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Synonyms

FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

Materials Supplied

Number Component 50T Concentration Storage
1 PBS Buffer (powder) 2L x 2 20x RT
2 Antigen Retrieval Buffer 20ml 100x 2-8°C
3 Endogenous Peroxidase Blocking Buffer 3ml RTU 2-8°C
4 Blocking Buffer 3ml RTU 2-8°C
5 Primary Antibody (Human Vimentin Mouse mAb) 6ml RTU 2-8°C
6 Secondary Antibody (HRP-Goat anti-Mouse IgG pAb) 6ml RTU 2-8°C
7 Chromogen Component A 0.3ml RTU -20°C
8 Chromogen Component B 0.3ml RTU -20°C
9 Counter Staining Reagent 5ml RTU RT
10 Differentiation Reagent 6ml RTU RT
11 Mounting Media 5ml RTU RT
12 Control slide (Human cervical carcinoma) 1 slide RTU RT
13 Datasheet 1 copy

Notes

  1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
  2. Do not allow slides to dry at any time during this procedure.
  3. Please don't replace the matching reagents in this product with other manufacturers' products.
  4. As DAB is a carcinogen, please take necessary precautions.
  5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

Please cite this product as "IHC0113H, Bioss Antibodies".
Citation example: “Human tissue sections using Human Vimentin IHC Kit (IHC0113H, Bioss Antibodies) were stained for Vimentin according to the manufacturer's instructions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.