Human CD31 Ready-To-Use IHC KitCat. No.: IHC0115H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
Platelet endothelial cell adhesion molecule precursor-1; PECAM-1; PECAM1; Adhesion molecule; CD31 antigen; CD31 EndoCAM; Endocam; FLJ34100; FLJ58394; GPIIA; Pecam 1; PECA1_HUMAN; PECAM 1 CD31 EndoCAM; PECA1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; Adhesion molecule; Platelet/endothelial cell adhesion molecule.
|1||PBS Buffer (powder)||2L x 2||20x||RT|
|2||Antigen Retrieval Buffer||20ml||100x||2-8°C|
|3||Endogenous Peroxidase Blocking Buffer||3ml||RTU||2-8°C|
|5||Primary Antibody (Human CD31 Mouse mAb)||6ml||RTU||2-8°C|
|6||Secondary Antibody (HRP-Goat anti-Mouse IgG pAb)||6ml||RTU||2-8°C|
|7||Chromogen Component A||0.3ml||RTU||-20°C|
|8||Chromogen Component B||0.3ml||RTU||-20°C|
|9||Counter Staining Reagent||5ml||RTU||RT|
|12||Control slide (Human tonsil)||1 slide||RTU||RT|
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
Please cite this product as " IHC0115H, Bioss Antibodies".
Citation example: “Human tissue sections using Human CD31 IHC Kit (IHC0115H, Bioss Antibodies) were stained for CD31 according to the manufacturer's instructions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.