Mouse SOX2 Ready-To-Use IHC Kit
Mouse SOX2 Ready-To-Use IHC Kit
Cat. No.: IHC0210MSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
Synonyms
ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb.
Materials Supplied
Number | Component | 50T | Concentration | Storage |
---|---|---|---|---|
1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
4 | Blocking Buffer | 3ml | RTU | 2-8°C |
5 | Primary Antibody (Mouse SOX2 Recombinant Rabbit mAb) | 6ml | RTU | 2-8°C |
6 | Secondary Antibody (Goat Anti-Rabbit IgG H&L / HRP) | 6ml | RTU | 2-8°C |
7 | Chromogen Component A | 0.3ml | RTU | -20°C |
8 | Chromogen Component B | 0.3ml | RTU | -20°C |
9 | Counter Staining Reagent | 5ml | RTU | RT |
10 | Differentiation Reagent | 6ml | RTU | RT |
11 | Mounting Media | 5ml | RTU | RT |
12 | Control slide (Mouse brain) | 1 slide | RTU | RT |
13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.