Human NCAM Ready-To-Use IHC Kit

Cat. No.: IHC0213H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.

Synonyms

CD56; E-NCAM; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC; CD56 antigen; Cell Adhesion Molecule Neural 1; N CAM 120; N CAM 180; NCAM 1; NCAM 120; NCAM 140.

Materials Supplied

Number	Component	50T	Concentration	Storage
1	PBS Buffer (powder)	2L x 2	20x	RT
2	Antigen Retrieval Buffer	20ml	100x	2-8°C
3	Endogenous Peroxidase Blocking Buffer	3ml	RTU	2-8°C
4	Blocking Buffer	3ml	RTU	2-8°C
5	Primary Antibody (Human NCAM Recombinant Rabbit mAb)	6ml	RTU	2-8°C
6	Secondary Antibody (Goat Anti-Rabbit IgG H&L / HRP)	6ml	RTU	2-8°C
7	Chromogen Component A	0.3ml	RTU	-20°C
8	Chromogen Component B	0.3ml	RTU	-20°C
9	Counter Staining Reagent	5ml	RTU	RT
10	Differentiation Reagent	6ml	RTU	RT
11	Mounting Media	5ml	RTU	RT
12	Control slide (Human cerebellum)	1 slide	RTU	RT
13	Datasheet	1 copy

Notes

1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
2. Do not allow slides to dry at any time during this procedure.
3. Please don't replace the matching reagents in this product with other manufacturers' products.
4. As DAB is a carcinogen, please take necessary precautions.
5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.