Rat SOX9 Ready-To-Use IHC Kit

Cat. No.: IHC0359R
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

Synonyms

2010306G03Rik, AV220920, CMD1, CMPD1, mKIAA4243, Sox-9, SOX9, SRA1, SRXX2, SRXY10.Validation Data

Materials Supplied

Number	Component	50T	Concentration	Storage
1	PBS Buffer (powder)	2L x 2	20x	RT
2	Antigen Retrieval Buffer	20ml	100x	2-8°C
3	Endogenous Peroxidase Blocking Buffer	3ml	RTU	2-8°C
4	Blocking Buffer	3ml	RTU	2-8°C
5	Primary Antibody (Rat SOX9 Recombinant Rabbit mAb)	6ml	RTU	2-8°C
6	Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated)	6ml	RTU	2-8°C
7	Chromogen Component A	0.3ml	RTU	-20°C
8	Chromogen Component B	0.3ml	RTU	-20°C
9	Counter Staining Reagent	5ml	RTU	RT
10	Differentiation Reagent	6ml	RTU	RT
11	Mounting Media	5ml	RTU	RT
12	Control slide (Rat testis)	1 slide	RTU	RT
13	Datasheet	1 copy

Notes

1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
2. Do not allow slides to dry at any time during this procedure.
3. Please don't replace the matching reagents in this product with other manufacturers' products.
4. As DAB is a carcinogen, please take necessary precautions.
5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.