Human Phospho-MDM2 (Ser166) Ready-To-Use IHC Kit
Human Phospho-MDM2 (Ser166) Ready-To-Use IHC Kit
Cat. No.: IHC0548HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.
Synonyms
Materials Supplied
| Number | Component | 50T | Concentration | Storage |
|---|---|---|---|---|
| 1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
| 2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
| 3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
| 4 | Blocking Buffer | 3ml | RTU | 2-8°C |
| 5 | Primary Antibody (Human Phospho-MDM2 (Ser166) Recombinant Rabbit mAb) | 6ml | RTU | 2-8°C |
| 6 | Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated) | 6ml | RTU | 2-8°C |
| 7 | Chromogen Component A | 0.3ml | RTU | -20°C |
| 8 | Chromogen Component B | 0.3ml | RTU | -20°C |
| 9 | Counter Staining Reagent | 5ml | RTU | RT |
| 10 | Differentiation Reagent | 6ml | RTU | RT |
| 11 | Mounting Media | 5ml | RTU | RT |
| 12 | Control slide (Human colon cancer) | 1 slide | RTU | RT |
| 13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Powered by Bioz