Mouse IFABP Ready-To-Use IHC Kit

Cat. No.: IHC0632M
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

The FABP2 gene encodes the intestinal fatty acid-binding protein 2 (I-FABP), which plays a significant role in lipid metabolism by facilitating the transport and intracellular trafficking of long-chain fatty acids in enterocytes, the absorptive cells in the intestine. FABP2 is involved in the uptake, metabolism, and transfer of fatty acids across cellular membranes, contributing to digestion and absorption processes in the intestine. This protein is crucial for efficient lipid metabolism, influencing fatty acid oxidation, and energy balance. Variations in the FABP2 gene have been associated with metabolic traits, such as insulin sensitivity, lipid profiles, and obesity, suggesting its impact on metabolic health. FABP2's function in intestinal lipid absorption and metabolism makes it a subject of interest in studies related to dietary effects on health and the development of metabolic disorders. Understanding FABP2's role is essential for insights into nutritional influences on health and possible therapeutic targets for metabolic diseases.

Synonyms

FABP2; FABPI; I-FABP; FABP 2; Fatty acid binding protein 2 intestinal; Fatty acid binding protein intestinal; Fatty acid-binding protein; IFABP; Intestinal fatty acid binding protein 2; MGC133132; FABPI_HUMAN.Validation Data

Materials Supplied

Number	Component	50T	Concentration	Storage
1	PBS Buffer (powder)	2L x 2	20x	RT
2	Antigen Retrieval Buffer	20ml	100x	2-8°C
3	Endogenous Peroxidase Blocking Buffer	3ml	RTU	2-8°C
4	Blocking Buffer	3ml	RTU	2-8°C
5	Primary Antibody (Mouse IFABP Recombinant Rabbit mAb)	6ml	RTU	2-8°C
6	Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated)	6ml	RTU	2-8°C
7	Chromogen Component A	0.3ml	RTU	-20°C
8	Chromogen Component B	0.3ml	RTU	-20°C
9	Counter Staining Reagent	5ml	RTU	RT
10	Differentiation Reagent	6ml	RTU	RT
11	Mounting Media	5ml	RTU	RT
12	Control slide (Mouse colon)	1 slide	RTU	RT
13	Datasheet	1 copy

Notes

1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
2. Do not allow slides to dry at any time during this procedure.
3. Please don't replace the matching reagents in this product with other manufacturers' products.
4. As DAB is a carcinogen, please take necessary precautions.
5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.