Human 14-3-3 alpha/beta/zeta/delta Ready-To-Use IHC Kit

Cat. No.: IHC0674H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

Synonyms

Materials Supplied

Number	Component	50T	Concentration	Storage
1	PBS Buffer (powder)	2L x 2	20x	RT
2	Antigen Retrieval Buffer	20ml	100x	2-8°C
3	Endogenous Peroxidase Blocking Buffer	3ml	RTU	2-8°C
4	Blocking Buffer	3ml	RTU	2-8°C
5	Primary Antibody (Human 14-3-3 alpha/beta/zeta/delta Recombinant Rabbit mAb)	6ml	RTU	2-8°C
6	Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated)	6ml	RTU	2-8°C
7	Chromogen Component A	0.3ml	RTU	-20°C
8	Chromogen Component B	0.3ml	RTU	-20°C
9	Counter Staining Reagent	5ml	RTU	RT
10	Differentiation Reagent	6ml	RTU	RT
11	Mounting Media	5ml	RTU	RT
12	Control slide (Human mammary gland)	1 slide	RTU	RT
13	Datasheet	1 copy

Notes

1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
2. Do not allow slides to dry at any time during this procedure.
3. Please don't replace the matching reagents in this product with other manufacturers' products.
4. As DAB is a carcinogen, please take necessary precautions.
5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.