RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Immunology

Immunology

Our Immunology Research Area is built to support the study of tumor biology, cancer signaling, biomarker discovery, and therapeutic target analysis across a broad range of experimental workflows. This collection includes antibodies for use in WB, IHC, ICC, IF, FC, ELISA, and other common applications.



×

Collection Menu
Lane 1: Human Jurkat cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Lymph node lysates probed with Beclin 1 Monoclonal Antibody, Unconjugated (bsm-33323M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of bsm-33323M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of bsm-33323M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of bsm-33323M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining

Catalog #1: bsm-33323m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-TNF-alpha Polyclonal Antibody, FITC conjugated (bsm-0387M-FITC) at 1:200 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded:human colon labeled with Anti-TNF-Alpha Polyclonal Antibody, Unconjugated (bsm-0387M), followed by conjugation to the secondary antibody and DAB staining
4% Paraformaldehyde-fixed THP-1(LPS treated (100 ng\/ml, 16 h) and Brefeldin A treated (5 \u00b5g\/ml, 4 h)) (H) cell; Antibody incubation with (TNF alpha) monoclonal Antibody, unconjugated (bsm-0387M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Blank control:THP-1. Primary Antibody (green line): Mouse Anti-TNF alpha antibody (bsm-0387M) Dilution: 1ug\/Test; Secondary Antibody : Goat anti-mouse IgG-FITC Dilution: 0.5ug\/Test. Protocol The cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bsm-0387m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0855m-cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33323m-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-0387m-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0387m-biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0855m

Applications: IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0855m-bf555

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33323m-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33323m-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33323m-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33323m-biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33323m-hrp

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33323m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33323m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33323m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33323m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33323m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33323m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33323m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33323m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33323m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33323m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33323m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33323m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33323m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33323m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33323m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33323m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33323m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33323m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33323m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-0387m-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-0387m-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-0387m-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-0387m-hrp

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0855m-cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-0855m-cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-0855m-fitc

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0855m-biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0855m-hrp

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0387m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-0855m-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-0387m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-0387m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0387m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0387m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0387m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555