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Immunology

Immunology
Our list of Immunology antibodies has been created for your convenience. For the month of August, the Immunology Collection will be discounted 15% off in support of Immunization Awareness month.
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human Siha cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HepG2 cell lysates probed with CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD14) Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit

Conjugate: Unconjugated

Lane 1: human colon carcinoma lysates probed with Anti phospho-ERK1\/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 44kD
Formalin-fixed and paraffin embedded human liver labeled with Anti-phospho-ERK1\/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ERK1\/2(Thr202 + Tyr204)Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3016R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Unconjugated

L1 mouse brain lysates L2 human colon carcinoma lysates probed with Anti - phospho-JNK1\/2\/3 (Thr183+Tyr185) Polyclonal Antibody, Unconjugated (bs-1640R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-JNK1+2+3 (THR183+TYR185) at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Mouse splenocytes probed with Rabbit Anti-JNK1\/2\/3 (Thr183+Tyr185) Polyclonal Antibody, Unconjugated (bs-1640R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1+2+3 (T183+Y185) Polyclonal Antibody, Unconjugated (bs-1640R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1640R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-1640R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy3

Catalog #1: bs-1640R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy5

Catalog #1: bs-1640R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy7

Catalog #1: bs-1640R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: FITC

Catalog #1: bs-1640R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Biotin

Catalog #1: bs-1640R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: HRP

Catalog #1: bs-3016R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy3

Catalog #1: bs-3016R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy5

Catalog #1: bs-3016R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy7

Catalog #1: bs-3016R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: FITC

Catalog #1: bs-3016R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Biotin

Catalog #1: bs-3016R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: HRP

Catalog #1: bs-1640R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-3016R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy5.5

Catalog #1: bs-1640R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE

Catalog #1: bs-3016R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE

Catalog #1: bs-1640R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 350

Catalog #1: bs-1640R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 405

Catalog #1: bs-1640R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-1640R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 555

Catalog #1: bs-1640R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 594

Catalog #1: bs-1640R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 647

Catalog #1: bs-1640R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 680

Catalog #1: bs-1640R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: AbBy Fluor® 750

Catalog #1: bs-1640R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: APC

Catalog #1: bs-1640R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: APC-Cy5

Catalog #1: bs-1640R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: APC-Cy5.5

Catalog #1: bs-1640R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: APC-Cy7

Catalog #1: bs-1640R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE-Cy3

Catalog #1: bs-1640R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE-Cy5

Catalog #1: bs-1640R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE-Cy5.5

Catalog #1: bs-1640R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE-Cy7

Catalog #1: bs-1640R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PerCP

Catalog #1: bs-1640R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PerCP-Cy5.5

Catalog #1: bs-1640R-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PerCP-Cy7

Catalog #1: bs-1640R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: RBITC

Catalog #1: bs-3016R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-3016R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-3016R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bs-3016R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bs-3016R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bs-3016R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bs-3016R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bs-3016R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor® 750

Catalog #1: bs-3016R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: APC