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Immunology

Immunology
Our list of Immunology antibodies has been created for your convenience. For the month of August, the Immunology Collection will be discounted 15% off in support of Immunization Awareness month.


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Catalog #1: bs-2379R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2646R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-2934R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombined Trx\/His-tag porcine GM-CSF protein; Lane 2: Recombined His-tag porcine GM-CSF protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3790r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Pig

Conjugate: Unconjugated

Catalog #1: bs-4790R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-6464R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-6416r-fitc-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-6761R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-TNF-alpha Polyclonal Antibody, FITC conjugated (bsm-0387M-FITC) at 1:200 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded:human colon labeled with Anti-TNF-Alpha Polyclonal Antibody, Unconjugated (bsm-0387M), followed by conjugation to the secondary antibody and DAB staining
4% Paraformaldehyde-fixed THP-1(LPS treated (100 ng\/ml, 16 h) and Brefeldin A treated (5 \u00b5g\/ml, 4 h)) (H) cell; Antibody incubation with (TNF alpha) monoclonal Antibody, unconjugated (bsm-0387M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Blank control:THP-1. Primary Antibody (green line): Mouse Anti-TNF alpha antibody (bsm-0387M) Dilution: 1ug\/Test; Secondary Antibody : Goat anti-mouse IgG-FITC Dilution: 0.5ug\/Test. Protocol The cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bsm-0387m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0648r-hrp-100ul

Applications: IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2616R-PE

Applications: WB, FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bs-0648r-bf488-100ul

Applications: FCM, IF(IHC-P), Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bs-0648r-bf647-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bs-0056R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0056R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-0647R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: FITC

Catalog #1: bs-0647R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Biotin

Catalog #1: bs-0577R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy3

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: human colon carcinoma lysate; Lane 2: rat brain lysate probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: MCF-7 cell lysates probed with TRADD Polyclonal Antibody, Unconjugated (bs-1202R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1202R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1240R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-1305R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-1444R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 Polyclonal Antibody, Unconjugated (bs-1703R) followed by conjugation to the secondary antibody and DAB staining
Images provided the Independent Validation Program (badge number 29651)Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti-c-Raf\/Raf1 Polyclonal Antibody (bs-1703R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Catalog #1: bs-1703R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1629R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2081R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-1970R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-1970R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2379R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2210R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: FITC

Catalog #1: bs-2605R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-2609R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2646R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MAPKAP Kinase 2 Polyclonal Antibody, Unconjugated (bs-2908R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC996 cells probed with Rabbit Anti-MAPKAP Kinase 2 Polyclonal Antibody (bs-2908R) at 1:10 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MAPKAP Kinase 2 Polyclonal Antibody, Unconjugated (bs-2908R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MAPKAP Kinase 2) Polyclonal Antibody, Unconjugated (bs-2908R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2908R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nPLCG 2 Polyclonal Antibody(bs-3532R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PLCG 2 Polyclonal Antibody, Unconjugated (bs-3532R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3532R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3864R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4755R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-4819R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-4682R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4914R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-6030R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-6416r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-8613R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0855m-cy3-100ul

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0195R-FITC

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0648r-cy5-100ul

Applications: FCM, IF(IHC-P), Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0648r-pe-100ul

Applications: FCM, IF(IHC-P), Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-2934R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0637R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC