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KD Validated Antibodies and Controls

KD Validated Antibodies and Controls

Bioss’ recombinant antibodies with knockdown validation are paired with their corresponding cell lysates to streamline your research and support reliable, reproducible results, making them ideal for a wide range of immunological applications.



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Catalog #1: bsm-90428r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90425r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90429r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90431r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90432r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90434r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90437r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90438r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90433r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90435r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90439r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90440r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90443r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Immunocytochemical staining of Hela cells with anti-Phospho-Paxillin (Y118) antibody (1:1,000). Nuclei were stained blue with DAPI; Phospho-Paxillin (Y118) was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.
Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.
Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Phospho-Paxillin (Y118) expression in wild type (WT) and Phospho-Paxillin (Y118) shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulinserves as a loading control. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Catalog #1: bsm-90447r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Protein arginine methyltransferase 5 expression in HeLa cells using Protein arginine methyltransferase 5 antibody (1:2,000). Green, isotype control; red, Protein arginine methyltransferase 5.
Immunocytochemical staining of Hela cells with anti-Protein arginine methyltransferase 5 antibody (1:1,000). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 5 was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 \u03bcm.
Western blotting analysis using anti-Protein arginine methyltransferase 5 antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Protein arginine methyltransferase 5 antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.
Western blotting analysis using anti-protein arginine methyltransferase 5 antibody. Protein arginine methyltransferase 5 expression in wild-type (WT) and protein arginine methyltransferase 5 (PRMT5) shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulin serves as a loading control. The blot was incubated with anti-protein arginine methyltransferase 5 antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Catalog #1: bsm-90445r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90451r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of RALBP1 expression in HeLa cells using RALBP1 antibody (1:2,000). Green, isotype control; red, RALBP1.
Immunocytochemical staining of Hela cells with anti-RALBP1 antibody (1:1,000). Nuclei were stained blue with DAPI; RALBP1 was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 \u03bcm.
Western blotting analysis using anti-RALBP1 antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RALBP1 antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RALBP1 antibody. RALBP1 expression in wild type (WT) and RALBP1 shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulin serves as a loading control. The blot was incubated with anti-RALBP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Catalog #1: bsm-90446r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Peroxiredoxin 6 expression in HepG2 cells using anti-Peroxiredoxin 6 antibody (1:2,000). Green, isotype control; red, Peroxiredoxin 6.
Immunocytochemical staining of HepG2 cells with anti-Peroxiredoxin 6 antibody (1:1,000). Nuclei were stained blue with DAPI; Peroxiredoxin 6 was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 \u03bcm.
Western blotting analysis using anti-Peroxiredoxin 6 antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peroxiredoxin 6 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.
Western blotting analysis using anti-Peroxiredoxin 6 antibody. Peroxiredoxin 6 expression in wild type (WT) and Peroxiredoxin 6 shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulin serves as a loading control. The blot was incubated with anti-Peroxiredoxin 6 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Catalog #1: bsm-90449r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Flow cytometric analysis of PSIP1 expression in HeLa cells using PSIP1 antibody (1:2,000). Green, isotype control; red, PSIP1.
Immunocytochemical staining of Hela cells with anti-PSIP1 antibody (1:1,000). Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 \u03bcm.
Western blotting analysis using anti-PSIP1 antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSIP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.
Western blotting analysis using anti-PSIP1 antibody. PSIP1 expression in wild-type (WT) and PSIP1 shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulin serves as a loading control. The blot was incubated with anti-PSIP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Catalog #1: bsm-90444r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90452r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90461r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90463r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90469r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90464r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90458r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90460r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90473r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90467r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90472r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90475r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90456r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90470r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90466r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90471r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90465r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90479r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90480r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90481r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90474r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90483r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90478r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90484r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90487r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90491r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90459r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90486r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90492r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90488r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated