Bromocresol Green

Overview
Catalog #	bs-75954c-25g
Product Name	Bromocresol Green
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at RT.
Target
Product Information	CAS Number: 76-60-8 Molecular formula: C21H14Br4O5S Molecular weight: 698.01 Purity: >95% (Dye content) Appearance: Beige to yellow-brown powder. Solubility: Soluble in ethanol (30mg/ml). InChiKey: FRPHFZCDPYBUAU-UHFFFAOYSA-N SMILES: O=S1(C2=CC=CC=C2C(C3=CC(Br)=C(O)C(Br)=C3C)(C4=C(C)C(Br)=C(O)C(Br)=C4)O1)=O
Description	Bromocresol green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualization of the compounds with functional groups whose pKa is below 5.0. One major use is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue). Visual transition interval: pH 3.8 (yellow) - 5.4 (blue). UV-Visible (lambdamax): 423 nm, 444 nm, 617 nm. Bromocresol green is also an inhibitor of prostaglandin uptake that blocks the immediate rise in intracellular immunoreactive PGE2 following treatment with 16,16-dimethyl-PGE2. Bromocresol green prevents the stimulatory effect of 16,16-dimethyl-PGE on cell proliferation, adhesion, migration and invasion and on HIF-1alpha expression and activity. Additional applications include use in sol-gel matrices and the detection of ammonia.