Fluorescein dioctanoate

Overview
Catalog #	bs-76327c-100mg
Product Name	Fluorescein dioctanoate
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at +4°C.
Target
Product Information	CAS Number: 19722-86-2 Molecular formula: C36H40O7 Molecular weight: 584.72 Purity: >98% Appearance: Off-white low melting solid. Solubility: Soluble in DMSO (5mg/ml). InChiKey: UWZNXQHGTKMTSX-UHFFFAOYSA-N SMILES: O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(OC(CCCCCCC)=O)C=C4OC5=C3C=CC(OC(CCCCCCC)=O)=C5
Description	Fluorescein dioctanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dioctanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dioctanoate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dioctanoate is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: lambdaEx 497nM, lambdaEm 516nm. Extinction coefficient: 80000 cm-1 M-1.