Resorufin-isobutyrate

Overview
Catalog #	bs-76447c-1mg
Product Name	Resorufin-isobutyrate
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 251292-24-7 Molecular formula: C17H15NO5 Molecular weight: 313.3 Purity: >97% (HPLC) Appearance: Solid. Solubility: Soluble in DMSO. InChiKey: RVDFSVVDUVHHKE-UHFFFAOYSA-N SMILES: CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2 SMILES: CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2
Description	Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission max. (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.