Indo 1-AM

Overview
Catalog #	bs-76451c-1mg
Product Name	Indo 1-AM
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 112926-02-0 Molecular formula: C47H51N3O22 Molecular weight: 1009.93 Purity: >90% (HPLC) Appearance: Off-white solid. Solubility: Soluble in DMSO or methanol. InChiKey: CAWBRCOBJNWRLK-UHFFFAOYSA-N SMILES: CC1=CC(OCCOC2=CC(C3=CC(C=CC(C(OCOC(C)=O)=O)=C4)=C4N3)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1 SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=CC3=C(N2)C=C(C=C3)C(=O)OCOC(C)=O)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1
Description	INDO 1-AM is a popular non-invasive UV-excitable calcium indicator. It is the cell-permeable ester derivative of INDO 1. After crossing the cell membrane, INDO 1-AM is rapidly hydrolyzed by cytoplasmic esterases to produce the ratiometric fluorescent calcium indicator INDO 1, which remains trapped within the cell. INDO 1/AM has been used to selectively monitor Ca2+ levels in mitochondria and in the cytosol. INDO 1 is ideal for analyses using flow cytometry, as it uses a single excitation source (usually the 351-364nm spectral lines of the argon-ion laser). In contrast to FURA 1, INDO 1 has a dual emission peak. The emission of INDO 1 shifts to 400nm (when bound to Ca2+) from 480nm in Ca2+-free environments. INDO 1 is prone to photobleaching, which limits its usefulness in methods involving microscopy. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Spectral Data: lambdaex 330nm; lambdaem 450nm (with Calcium), lambdaex 356nm; lambdaem 478nm in methanol.