Nile Red

Overview
Catalog #	bs-76594c-1g
Product Name	Nile Red
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 7385-67-3 Molecular formula: C20H18N2O2 Molecular weight: 318.4 Purity: >98% (HPLC) Appearance: Green to brown powder. Solubility: Soluble in chloroform. InChiKey: VOFUROIFQGPCGE-UHFFFAOYSA-N SMILES: CCN(CC)C1=CC=C2N=C3C(OC2=C1)=CC(=O)C1=C3C=CC=C1 SMILES: CCN(CC)C1=CC=C2N=C3C(OC2=C1)=CC(=O)C1=CC=CC=C31
Description	Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm). Extinction coeff. 0.60 ± 0.05 (0.01g/l; 0,5cm).