TMB dihydrochloride

Overview
Catalog #	bs-76846c-1g
Product Name	TMB dihydrochloride
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at +4°C.
Target
Product Information	CAS Number: 64285-73-0 Molecular formula: C16H20N2 . 2HCl Molecular weight: 240.3 . 72.9 Purity: >95% (HPLC) Appearance: White to off-white to pink powder. Solubility: Soluble in DMSO. InChiKey: NYNRGZULARUZCC-UHFFFAOYSA-N SMILES: Cl[H].Cl[H].CC1=CC(=CC(C)=C1N)C1=CC(C)=C(N)C(C)=C1 SMILES: Cl.Cl.CC1=CC(=CC(C)=C1N)C1=CC(C)=C(N)C(C)=C1
Description	TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (lambdamax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.