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Loading Controls

Loading Controls

 

Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples.

Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.



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Catalog #1: bsm-0917m-cy3-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-0917m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-0917m-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-0917m-fitc-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-0917m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-0917m-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-0917m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BrdU (A7)) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

Catalog #1: bsm-0917m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-20496R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-20496R-Cy3

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-20496R-Cy5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-20496R-Cy5.5

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-20496R-Cy7

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-20496R-FITC

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-20496R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-8783r

Applications: WB

Reactivity: yeast

Conjugate: Unconjugated

Catalog #1: bs-20496r

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33036m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Catalog #1: bs-8537R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bs-8537R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bs-8537R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bs-8537R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bs-8537R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bs-8537R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bs-8537R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bs-2007R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-2007R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2007R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-2007R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-2007R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-2007R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-2007R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-2006R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-2006R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2006R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-2006R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-2006R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-2006R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-2006R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-24447R-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-24447R-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-24447R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-24447R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-24447R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-24447R-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-24447R-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33235m-rbitc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: RBITC

Catalog #1: bsm-33235m-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PerCP-Cy5.5